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  Indian J Med Microbiol
 

Figure 7: Testing the %efficiency for the primers and two housekeeping genes. The order of each primers for real-time polymerase chain reaction as follows: Row1 (B2–B7): hepatocyte nuclear factor 1, Row 2 (C2–C7): PROS1, Row 3 (E1–E7): YWHAZ and Row 4 (F1–F7): UBC, The last column (7) contains only double distilled water as negative control for all the primers and the first column (2) has a neat volume for each gene

Figure 7: Testing the %efficiency for the primers and two housekeeping genes. The order of each primers for real-time polymerase chain reaction as follows: Row1 (B2–B7): hepatocyte nuclear factor 1, Row 2 (C2–C7): PROS1, Row 3 (E1–E7): YWHAZ and Row 4 (F1–F7): UBC, The last column (7) contains only double distilled water as negative control for all the primers and the first column (2) has a neat volume for each gene