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  Indian J Med Microbiol
 

Figure 6: The order of each housekeeping gene primer in real-time polymerase chain reaction wells. Columns 5 and 6 present wells contain negative controls (shaded in green), which consist of 10.5 double distilled water, 1 mL of each primer (forward and reverse) and 12.5 mL SYBR-490. Each row contains 1 mL of each primer (forward and reverse) columns 2–4 (and 2 mL cDNA, 8.5 mL double distilled water and 12.5 mL SYBR green): A: Hepatocyte nuclear factor 1; B: PROS1; C: B2M1; D: HPRT1; E: RPL13A; F: SDHA; G: UBC; H: YWHAZ

Figure 6: The order of each housekeeping gene primer in real-time polymerase chain reaction wells. Columns 5 and 6 present wells contain negative controls (shaded in green), which consist of 10.5 double distilled water, 1 mL of each primer (forward and reverse) and 12.5 mL SYBR-490. Each row contains 1 mL of each primer (forward and reverse) columns 2–4 (and 2 mL cDNA, 8.5 mL double distilled water and 12.5 mL SYBR green): A: Hepatocyte nuclear factor 1; B: PROS1; C: B2M1; D: HPRT1; E: RPL13A; F: SDHA; G: UBC; H: YWHAZ