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  Indian J Med Microbiol
 

Figure 29: The 100% efficiency results (melting curves) for hepatocyte nuclear factor 1, PROS1 and two housekeeping genes: YMHAZ and UBC. D2: neat volume of YWHAZ D3–D6: YMHAZ in different concentration: D3: 10−1, D4: 10−2, D5: 10−3 and D6: 10−4. D7: Double distilled water as negative control; Tm for D2 and D3 = 83; Tm for D4–D6 = 82

Figure 29: The 100% efficiency results (melting curves) for hepatocyte nuclear factor 1, PROS1 and two housekeeping genes: YMHAZ and UBC. D2: neat volume of YWHAZ D3–D6: YMHAZ in different concentration: D3: 10<sup>−1</sup>, D4: 10<sup>−2</sup>, D5: 10<sup>−3</sup> and D6: 10<sup>−4</sup>. D7: Double distilled water as negative control; Tm for D2 and D3 = 83; Tm for D4–D6 = 82