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  Indian J Med Microbiol
 

Figure 16: The 100% efficiency results (melting curves) for hepatocyte nuclear factor 1, PROS1 and two housekeeping genes: YMHAZ and UBC E2: neat volume of YMHAZ. E3–E6: UBC in different concentration: E3: 10−1, E4: 10−2, E5: 10−3 and E6: 10−4. E7: Double distilled water as negative control; Tm for E2 = 89; Tm for E3 and E4 = 80; Tm for E5 = 81; Tm for E6 = 82

Figure 16: The 100% efficiency results (melting curves) for hepatocyte nuclear factor 1, PROS1 and two housekeeping genes: YMHAZ and UBC E2: neat volume of YMHAZ. E3–E6: UBC in different concentration: E3: 10<sup>−1</sup>, E4: 10<sup>−2</sup>, E5: 10<sup>−3</sup> and E6: 10<sup>−4</sup>. E7: Double distilled water as negative control; Tm for E2 = 89; Tm for E3 and E4 = 80; Tm for E5 = 81; Tm for E6 = 82