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  Indian J Med Microbiol
 

Figure 15: The 100% efficiency results (melting curves) for hepatocyte nuclear factor 1, PROS1 and two housekeeping genes: YMHAZ and UBC. C2: neat volume of PROS1. C3–C6: Different concentration of PROS1 C3: 10−1, C4: 10−2, C5: 10−3 and C6: 10−4. C7: Double distilled water as negative control; Tm for C2–C5 = 80; Tm for C6 = 77

Figure 15: The 100% efficiency results (melting curves) for hepatocyte nuclear factor 1, PROS1 and two housekeeping genes: YMHAZ and UBC. C2: neat volume of PROS1. C3–C6: Different concentration of PROS1 C3: 10<sup>−1</sup>, C4: 10<sup>−2</sup>, C5: 10<sup>−3</sup> and C6: 10<sup>−4</sup>. C7: Double distilled water as negative control; Tm for C2–C5 = 80; Tm for C6 = 77