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  Indian J Med Microbiol
 

Figure 14: The 100% efficiency results (melting curves) for hepatocyte nuclear factor 1, PROS1 and two housekeeping genes: YMHAZ and UBC. B2: neat volume of hepatocyte nuclear factor 1 B3–B6: Different concentration of hepatocyte nuclear factor 1: B3: 10−1, B4: 10−2, B5: 10−3 and B6: 10−4. B7: Double distilled water as negative control; PDs: Primer dimers; melting temperature (Tm) for B2–B4 = 87;

Figure 14: The 100% efficiency results (melting curves) for hepatocyte nuclear factor 1, PROS1 and two housekeeping genes: YMHAZ and UBC. B2: neat volume of hepatocyte nuclear factor 1 B3–B6: Different concentration of hepatocyte nuclear factor 1: B3: 10<sup>−1</sup>, B4: 10<sup>−2</sup>, B5: 10<sup>−3</sup> and B6: 10<sup>−4</sup>. B7: Double distilled water as negative control; PDs: Primer dimers; melting temperature (Tm) for B2–B4 = 87;