Close
  Indian J Med Microbiol
 

Figure 5: Mechanism of interference from Biotin in Competitive immunoassay (a) Competitive immunoassay without any interference from Biotin resulting in a true positive or true negative result; (b) High levels of biotin binds to the streptavidin sites preventing the complex formation between biotinylated antibody and labelled analyte analogue, leading to false high result

Figure 5: Mechanism of interference from Biotin in Competitive immunoassay (a) Competitive immunoassay without any interference from Biotin resulting in a true positive or true negative result; (b) High levels of biotin binds to the streptavidin sites preventing the complex formation between biotinylated antibody and labelled analyte analogue, leading to false high result