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  Indian J Med Microbiol
 

Figure 4: Mechanism of interference from Biotin in Sandwich immunoassay or Non-Competitive immunoassay. (a) Noncompetitive immunoassay without any interference from Biotin result in a true positive result; (b) High levels of biotin bind to the streptavidin sites preventing the complex formation between biotinylated antibody, labelled antibody and analyte leading to false low result

Figure 4: Mechanism of interference from Biotin in Sandwich immunoassay or Non-Competitive immunoassay. (a) Noncompetitive immunoassay without any interference from Biotin result in a true positive result; (b) High levels of biotin bind to the streptavidin sites preventing the complex formation between biotinylated antibody, labelled antibody and analyte leading to false low result