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  Indian J Med Microbiol
 

Figure 3: Mechanism of high-dose hook effect in sandwich immunoassay. As the analyte concentration increases the capture antibody and labelled antibody gets saturated with the analyte, thus decreasing the analyte sandwich formation. This results in an inappropriately low signal that causes erroneous low or normal result in spite of the sample containing elevated analyte concentration

Figure 3: Mechanism of high-dose hook effect in sandwich immunoassay. As the analyte concentration increases the capture antibody and labelled antibody gets saturated with the analyte, thus decreasing the analyte sandwich formation. This results in an inappropriately low signal that causes erroneous low or normal result in spite of the sample containing elevated analyte concentration