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  Indian J Med Microbiol
 

Figure 2: Mechanism of interference from endogenous antibodies in a sandwich or non-competitive immunoassay. (a) In the absence of interfering antibodies, capture antibody and labelled antibody target, two different epitopes on the analyte giving a true positive result; (b) Presence of interfering antibody can result in cross-linking of capture and labelled (detection) antibody giving a false-positive result; (c) Endogenous antibodies can bind to either capture or labelled antibody only, thus reducing sandwich formation giving a false-positive result

Figure 2: Mechanism of interference from endogenous antibodies in a sandwich or non-competitive immunoassay. (a) In the absence of interfering antibodies, capture antibody and labelled antibody target, two different epitopes on the analyte giving a true positive result; (b) Presence of interfering antibody can result in cross-linking of capture and labelled (detection) antibody giving a false-positive result; (c) Endogenous antibodies can bind to either capture or labelled antibody only, thus reducing sandwich formation giving a false-positive result