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  Indian J Med Microbiol
 

Figure 4: Effect of streptozotocin on the expression of marker proteins. 50 μg protein from control and streptozotocin-treated cellular extracts were separated on 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis gels and electroblotted onto nitrocellulose by Western blot technique. The antibody reacting bands were then visualized using specific antibodies for Hsp-70 and HO-1. Beta-actin was used as loading control. Results are representative of three experiments. The quantitation of protein bands is expressed as relative ratios normalized against the loading control, beta-actin. The asterisks (*) indicate significant difference (P ≤ 0.05) from the control cells treated with vehicle alone. Hsp-70: Heat shock protein 70, HO-1: Heme oxygenase-1

Figure 4: Effect of streptozotocin on the expression of marker proteins. 50 μg protein from control and streptozotocin-treated cellular extracts were separated on 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis gels and electroblotted onto nitrocellulose by Western blot technique. The antibody reacting bands were then visualized using specific antibodies for Hsp-70 and HO-1. Beta-actin was used as loading control. Results are representative of three experiments. The quantitation of protein bands is expressed as relative ratios normalized against the loading control, beta-actin. The asterisks (*) indicate significant difference (<i>P</i> ≤ 0.05) from the control cells treated with vehicle alone. Hsp-70: Heat shock protein 70, HO-1: Heme oxygenase-1