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  Indian J Med Microbiol
 

Figure 2: Effects of STZ on oxidative stress in Rin-5F cells and its attenuation by NAC. Rin-5F cells were treated with STZ in normal glucose medium and ROS (a) was measured by the 2',7'-dichlorofluorescein diacetate based assay to measure the intracellular production of ROS as described before. The results are mean ± standard error of the mean of three independent experiments. The asterisks (*) indicate significant difference (P ≤ 0.05) from the control cells treated with vehicle alone for 24 h, # indicates significant difference (P ≤ 0.05) from the control cells treated with vehicle alone for 48 h, δ indicates significant difference (P ≤ 0.05) from the STZ-treated cells at 24 h and ε indicates significant difference (P ≤ 0.05) from the STZ-treated cells at 48 h. DNA fragmentation (b) was analysed by agarose gel (2%) electrophoresis and ethidium bromide staining. Protein carbonylation (c) was assayed by the dinitrophenylhydrazine method. The asterisks indicate significant difference (**P ≤ 0.005, ***P ≤ 0.001) from the control cells treated with vehicle alone and δ indicates significant difference (P ≤ 0.05) from the STZ-treated cells at 24 h. NAC: N-acetyl cysteine, STZ: Streptozotocin, ROS: Reactive oxygen species

Figure 2: Effects of STZ on oxidative stress in Rin-5F cells and its attenuation by NAC. Rin-5F cells were treated with STZ in normal glucose medium and ROS (a) was measured by the 2',7'-dichlorofluorescein diacetate based assay to measure the intracellular production of ROS as described before. The results are mean ± standard error of the mean of three independent experiments. The asterisks (*) indicate significant difference (<i>P</i> ≤ 0.05) from the control cells treated with vehicle alone for 24 h, # indicates significant difference (<i>P</i> ≤ 0.05) from the control cells treated with vehicle alone for 48 h, δ indicates significant difference (<i>P</i> ≤ 0.05) from the STZ-treated cells at 24 h and ε indicates significant difference (<i>P</i> ≤ 0.05) from the STZ-treated cells at 48 h. DNA fragmentation (b) was analysed by agarose gel (2%) electrophoresis and ethidium bromide staining. Protein carbonylation (c) was assayed by the dinitrophenylhydrazine method. The asterisks indicate significant difference (**<i>P</i> ≤ 0.005, ***<i>P</i> ≤ 0.001) from the control cells treated with vehicle alone and δ indicates significant difference (<i>P</i> ≤ 0.05) from the STZ-treated cells at 24 h. NAC: N-acetyl cysteine, STZ: Streptozotocin, ROS: Reactive oxygen species